Practical Tips for Construction of Custom Peptide Libraries and Affinity Selection by Using Commercially Available Phage Display Cloning Systems
Figure 1
A typical procedure of the biopanning. (a) Incubation of phage library with an immobilized target. (b) Washing of unbound phage. (c) Elution of target-bound phage. (d) Amplification of the eluted phage for subsequent rounds of the biopanning.