Review Article
Practical Tips for Construction of Custom Peptide Libraries and Affinity Selection by Using Commercially Available Phage Display Cloning Systems
Table 1
Features of various systems of M13 and T7 phages.
| | System | Size limit | Numbers of peptides per virion | Presentation region |
| M13 | 3 | Unknown | 5 | N-terminus to gp3 | 33 | No limit | <1 | | 8 | Short | >2,700 | N-terminus to gp8 | 88 | Unknown | <300 | | No limit | 100–1000 | | Unknown | | C-terminus to gp8 | | No limit | <1 | N-terminus to gp6 | | C-terminus to gp6 | | | N-terminus to gp9 |
| T7 | T7Select1-1 | 1200aa | <1 | C-terminus to gp10B | T7Select1-2 | 900aa | T7Select10-3 | 1200aa | 5–15 | T7Select415-1 | 50aa | 415 |
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