Review Article
Practical Tips for Construction of Custom Peptide Libraries and Affinity Selection by Using Commercially Available Phage Display Cloning Systems
Table 4
Comparison of the M13 and T7 phage in library construction and affinity selection.
| | M13 phage | T7 Phage |
| Cost | Routinely cheap, requires | Routinely expensive, no additional | electroporator and cuvettes | instruments required | Site of library peptide | N-terminus of gp3 | C-terminus of gp10B | Library size (per g DNA) | ~109 | ~108 [2] | Peptide sequence bias | Highly biased | Less biased | Time required for phage tittering/amplification | Long | Short |
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