Chemical Approaches for Structure and Function of RNA in Postgenomic Era
Figure 1
Nuclear and nucleolar morphology. Normal rat liver nuclei have 1–6 round nucleoli which are less than 2 μm in diameter. In regenerating liver, cells contain enlarged nucleoli. In tumor cells (hepatocellular carcinoma), the nucleoli are not only enlarged but also they become pleomorphic in morphology. Nuclei were isolated by homogenization in 2.3–2.4 M sucrose containing 3.3 mM CaCl2. Nuclei were sonicated in 0.34 M sucrose and layered on 0.88 M sucrose for purification by centrifugation. Isolated nuclei and nucleoli had high purity, and morphologies were well preserved [6].
