Chemical Approaches for Structure and Function of RNA in Postgenomic Era
Figure 12
RNA 5′ and 3′ end labeling with [3H] by treatment with NaIO4 and [3H]-KBH4. The 4–7S RNA from Novikoff hepatoma cell nuclei was labeled to detect the presence of free 2′-OH and 3′-OH by NaIO4 oxidation followed by [3H]-KBH4 reduction. The reaction occurred at both ends of RNA (5′ end and 3′ end).
