Chemical Approaches for Structure and Function of RNA in Postgenomic Era

Figure 14

DEAE-Sephadex column chromatography of 4.5S RNA [38]. The 4.5S RNA from preparative gel electrophoresis was collected and chromatographed on a DEAE-Sephadex A-50 column. It was separated into 3 peaks but no radioactivity in 4.5S RNA III was detected, indicating the absence of accessible 2′-OH and 3′-OH in this molecule. The 4.5S RNA II may be the U6 RNA.