Chemical Approaches for Structure and Function of RNA in Postgenomic Era
Figure 15
DEAE-Sephadex chromatography of 5′ oligonucleotide [37]. The U1 RNA, U2 RNA, and U3 RNA collected from preparative gel electrophoresis (Figure 13) were digested with RNase A and subjected to DEAE-Sephadex A-25 column chromatography. The radioactive peaks at nucleoside region were coming from 3′ ends and the radioactivities at the regions of penta-, tetra-, and hexanucleotides were from 5′ end labeling. These fragments were treated with T1 RNase and found to be shortened by one nucleotide only in U3 5′ oligonucleotide indicating that G was next to the terminal pyrimidine nucleotide.
