Chemical Approaches for Structure and Function of RNA in Postgenomic Era
Figure 20
2D map of U1 RNA digested with T2 RNase and U2 RNase [16]. The U1 RNA uniformly labeled with [32P] was digested with T2 RNase and U2 RNase. The resistant 5′ fragment (spot “a”) was separated from the rest of the hydrolysate by two-dimensional electrophoresis. The first dimension was on cellogel at pH 3.5, and the second dimension was on DEAE paper in 5% acetic acid-NH4 acetate at pH 3.5.
