Chemical Approaches for Structure and Function of RNA in Postgenomic Era

Figure 28

The susceptibility of 5′ cap to pyrophosphatase [12]. The [³²P]-labeled 5′ oligonucleotide obtained from U2 RNA by RNase A was digested with pyrophosphatase and base composition was analyzed by snake venom phosphodiesterase digestion. This digestion released m_32,2,7G from the 5′ fragment indicating that m_32,2,7G is linked by a pyrophosphate linkage.