Chemical Approaches for Structure and Function of RNA in Postgenomic Era

Figure 30

m_32,2,7G^ identification from U3 RNA 5′ end fragment [58]. The [³H]-labeled U3 RNA 5′ fragment obtained by RNase T₁ and RNase A was digested with snake venom phosphodiesterase and alkaline phosphatase. The nucleoside mixture was separated by two-dimensional thin layer chromatography with standard nucleoside mixture in (a) and with the trialcohol derivative of m_32,2,7G^ in (b). The released nucleoside trialcohol derivative was identified as m_32,2,7G^ by fluorography.

(a)

(b)