Chemical Approaches for Structure and Function of RNA in Postgenomic Era

Figure 31

U3 RNA cap I and cap II chromatography [58]. The 5′ fragment from Figure 29 was eluted and treated with alkaline phosphatase and chromatographed on DEAE-Sephadex A-25 with standards of GMP, GDP, and GTP. Component “11B” was eluted in the GDP (cap I, m_32,2,7GpppAmA) region and “11A” (cap II, m_32,2,7GpppAmAmA) was eluted in the GTP region.