Chemical Approaches for Structure and Function of RNA in Postgenomic Era

Figure 7

The CMCT reaction of pseudouridine (Ψ) and uridine, and the structure of CMCT [11]. Adducts formed with CMCT on Ψ and U are shown. This adduct formation prevents the cleavage by RNase A at U but not at C. The mild alkaline treatment of reaction products destroys the U but not the Ψ. These differences were utilized to locate the position of Ψ by reverse transcriptase.