In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element against Clostridium difficile Toxin B and Sensitive Detection in Human Fecal Matter

Figure 5

Secondary structure and sequence of R12.69 ssDNA MRE. (a) ssDNA sequence of toxin B MRE R12.69. The red portions indicate the constant regions for primer attachment, and the black portion indicates the variable region. (b) Mfold prediction of R12.69 secondary structure. Highlighted sequence, CTAA, represents most common tetranucleotide sequence in the variable regions of the 43 sequences obtained from the round 12 library, and it is used as the center for sequence alignment in Figure 4 [33].

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