In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element against Clostridium difficile Toxin B and Sensitive Detection in Human Fecal Matter

Table 2

Cross binding reactivity of R12.69 ssDNA MRE.

Target	Average fluorescence (RFU)	Standard deviation	value	Selectivity ratio
Toxin B	0.0176	0.0066	—	—
Cholera toxin	0.0080	0.0041	0.0497	2.2
Alpha toxin	0.0033	0.0022	0.0117	5.4
Exotoxin A	0.0037	0.0022	0.0130	4.7
Bovine serum albumin	0.0030	0.0018	0.0106	5.8

For each protein target, average fluorescence is given with standard deviation. The value is given from a -test between toxin B and other negative targets. The selective ratio describes the number of times greater binding to toxin B than to other negative targets.