Exposure to a Pathological Condition May Be Required for the Cells to Secrete Exosomes Containing mtDNA Aberration

Figure 3

NSC exosome D-loop clone. (a) BLAST analysis against Homo sapiens mitochondrion, complete genome NC_012920.1. CD63+ exosomal mtDNA amplified with D-loop primers (location: 321-496). PCR products were cloned into the pCR4-TOPO-TA vector. (b) The red arrow in the sequence figure indicates the position of the nucleotide deletion in exosomal DNA in Homo sapiens mitochondrion complete genome sequence (NC_012920.1). The entire chromatogram of the clone is provided in the supplementary section (Figure S-7). (c) Singular SNP at 477 (A>-) found in the clone (bold letter in (a)) is also reported in MITOMAP at rCRS position 477 (T>-). Information from MITOMAP: the current GB frequency data is derived from two sets of human mitochondrial sequences from GenBank: 52,633 full-length (FL) sequences (>15.4 kbp) and 74,970 short, control region (CR) containing sequences (0.4-1.6 kbp); ^‡high-frequency haplogroups: variants found in haplogroups at 50% or higher are marked with a flag.

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