Exposure to a Pathological Condition May Be Required for the Cells to Secrete Exosomes Containing mtDNA Aberration

Table 2

A concise account of the SNP found in exosomal mtDNA. D-loop region 321-496 in GBM has a significantly higher occurrence of SNP over other regions and cell lines. Except for one SNP found at position 3545 (C>-) in NADH dehydrogenase subunit 1 region 3458-3561, the rest of the iPS-NSC and iPS-NSC-AD cell line clones had 100% identity to the reported sequences in the human mitochondrial genome (NC_012920.1) for all other genes. Each SNP in a clone is unique irrespective of the primer pair or a cell line.

Exosomal cell source	mtDNA region	Position on NC_012920.1	SNP
NSC	D-loop	321-496	477 A>-, 334 T>-
GBM	D-loop	321-496	371 G>N, 426 T>A, 420 G>N, 404 G>T, 481: G>N, 480: A>T, 478: T>N, 477: A>N and 379: T>C, 480: T>A
NSC	16S ribosomal RNA	1873-2078	1893 A>-, 1900 T>C
GBM	16S ribosomal RNA	1873-2078	1884 C>-
GBM	tRNA-Leu-UUR	3212-3319	None
NSC	tRNA-Leu-UUR	3212-3319	None
NSC	NADH dehydrogenase subunit 1	3458-3561	3502, T>A
iPS-NSC	NADH dehydrogenase subunit 1	3458-3561	3545 C>-