Synthesis and Evaluation of MGB Polyamide-Oligonucleotide Conjugates as Gene Expression Control Compounds

Table 2

values of modified dsDNAs and respective values.

dsDNAs	Complementary DNA		Mismatch DNA^d)
	-d(GCCTGGGACCG)-		-d(GCCTGaGACCG)-		-d(GCCTGGGACtG)-
	(°C) ^b)	(°C)^c)	(°C)^b)	(°C)^c)	(°C)^b)	(°C)^c)
Unmodified DNA	41.4	—	33.9	—	35.5	—
Modified DNA^a)
ON 3 ()	43.7	+2.3	35.4	+1.5	37.1	+1.6
ON 3 ()	46.5	+5.1
ON 3 ()	43.6	+2.2
ON 4 ()	63.3	+21.9	41.1	+7.2	55.8	+20.3
ON 4 ()	53.6	+12.2
ON 4 ()	49.8	+8.4

a) modified DNA: -d(CGGACCCTGGC)-, ON 3 (G = Im₃-NH(CH₂)_n-G); ON 4 (G = Py₃-γ-Im₃-NH(CH₂)_n-G). b) dsDNA (4.3 μM) in 10 mM sodium phosphate buffer (pH 7.0) containing 10 mM NaCl and 0.1 mM Na₂EDTA. c) ΔT_m (°C) = T_m (modified dsDNA)-T_m (unmodified dsDNA). d) mismatch base, a: adenine, t: thymine.