Combining Hyaluronic Acid with Chitosan Enhances Gene Delivery
Table 1
Composition, size, polydispersity, and zeta potential of CS and CSHA polyplexes.
Particle
HA (kDa)
CS : HA ratio
Size (, nm)
Polydispersity
Zeta potential (mV)
CS
−
—
—
402.8 ± 94.10
0.273 ± 0.128
19.9 ± 2.38
CSpDNA
+
—
—
241.8 ± 64.04****
0.266 ± 0.068
20.09 ± 2.28
CSHA1
−
132
5 : 1
351.3 ± 24.79##
0.245 ± 0.092
23.57 ± 1.19
CSHA2
+
328.1 ± 50.25
0.260 ± 0.088
24.18 ± 3.06
CSHA3
−
7 : 1
334.7 ± 62.76#
0.157 ± 0.066
18.20 ± 2.30
CSHA4
+
267.7 ± 30.52****
0.293 ± 0.080
19.08 ± 3.56
CSHA5
−
214
5 : 1
360.5 ± 69.66##
0.183 ± 0.064
24.29 ± 3.19
CSHA6
+
322.5 ± 69.94
0.270 ± 0.097
23.04 ± 2.31
CSHA7
−
7 : 1
345.6 ± 43.05#
0.243 ± 0.068
20.84 ± 4.48
CSHA8
+
284.9 ± 34.31***
0.298 ± 0.065
20.00 ± 2.60
or absence of pDNA in the formulation is indicated by + or −, respectively. Values marked with asterisks are statistically different to CS value, , . Values marked with cardinals are statistically different to CSDNA value, , (statistical differences determined by Tukey’s multiple comparisons test).
