UV Photocatalysis of Bone Marrow-Derived Macrophages on TiO₂ Nanotubes Mediates Intracellular Ca²⁺ Influx via Voltage-Gated Ca²⁺ Channels

Figure 2

UV-mediated photocatalysis of TiO₂ nanotubes elicits an increase in the concentration of cytosolic Ca²⁺ () and a decrease in the concentration of cytosolic reactive oxygen species () in BMMs, both of which are abolished by N-acetyl-L-cysteine (NAC) treatment. (a, b) Under continuous perfusion with HEPES buffer, cells seeded on the (a) polished Ti and (b) TiO₂ nanotubes were, respectively, exposed to UV light (wavelength = 340 nm and 380 nm). Following UV exposure, (red line) and (black line) levels were simultaneously measured and presented as described in “Section 2”. (c) The columns show the percentage of decrement compared to the initial intensity. (d) response in cells seeded on TiO₂ nanotubes was measured in the presence of 10 mM of NAC. NAC diluted in regular HEPES buffer was treated for the indicated time and washed out with regular HEPES buffer. (e) The columns show increment ().

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