Studies on the Genotoxicity Behavior of Silver Nanoparticles in the Presence of Heavy Metal Cadmium Chloride in Mice
Table 4
(a) Tail length, % DNA in tail, and tail moment in bone marrow cells of mice injected i.p. with Ag nanoparticles or/and CaCl2. (b) Tail length, % DNA in tail, and tail moment in testis of mice injected i.p. with Ag nanoparticles or/and CaCl2. (c) Tail length, % DNA in tail, and tail moment in sperms of mice injected i.p. with Ag nanoparticles or/and CaCl2.
(a)
Group
Treatment (dose mg/kg)
Tail length (px)
% DNA in tail
Tail moment
1
Negative control
7.60 ± 0.98
16.57 ± 1.33
1.29 ± 0.19
2
Cd(1.6)
25.30 ± 2.54
28.25 ± 1.58
7.47 ± 1.02
3
Ag(20)
15.50 ± 1.11
23.03 ± 1.94
3.82 ± 0.47
4
Ag(41)
24.77 ± 2.67
31.85 ± 3.74
8.20 ± 0.47
5
Ag(82)
31.62 ± 1.80
43.58 ± 2.44
13.64 ± 1.01
6
Ag(20) + Cd
26.67 ± 2.34
34.59 ± 2.98
9.17 ± 0.72
7
Ag(41) + Cd
39.02 ± 2.94
46.62 ± 1.67
18.15 ± 1.12
8
Ag(82) + Cd
50.02 ± 4.88
54.74 ± 3.18
27.16 ± 2.74
Groups 3, 4, and 5
= 84.4, < 0.001
= 67.2, < 0.001
= 194.0, < 0.001
Groups 6, 7, and 8
= 53.9, < 0.001
= 70.7, < 0.001
= 130.7, < 0.001
Results were expressed as mean ± SD. Statistically significantly different from the negative control group and statistically significantly different from the comparable nano-Ag treated group at < 0.001 using Student’s -test. One way ANOVA was used to test the effect of different doses of Ag nanoparticles on the tested parameters.
(b)
Group
Treatment (dose mg/kg)
Tail length (px)
% DNA in tail
Tail moment
1
Negative control
6.23 ± 0.51
14.14 ± 1.06
0.88 ± 0.11
2
Cd(1.6)
16.60 ± 2.20
25.11 ± 2.46
4.73 ± 0.96
3
Ag(20)
21.29 ± 2.41
22.11 ± 1.72
4.85 ± 0.99
4
Ag(41)
31.27 ± 3.16
32.99 ± 1.18
10.42 ± 1.30
5
Ag(82)
45.31 ± 0.75
44.79 ± 2.64
20.14 ± 1.47
6
Ag(20) + Cd
29.29 ± 2.04
33.68 ± 2.64
9.71 ± 0.57
7
Ag(41) + Cd
45.70 ± 3.56
48.45 ± 3.50
22.45 ± 3.16
8
Ag(82) + Cd
63.47 ± 3.48
71.39 ± 4.25
45.14 ± 3.03
Groups 3, 4, and 5
= 133.4, < 0.001
= 170.4, < 0.001
= 185.2, < 0.001
Groups 6, 7, and 8
= 151.5, < 0.001
= 145.2, < 0.001
= 248.8, < 0.001
Results were expressed as mean ± SD. Statistically significantly different from the negative control group and statistically significantly different from the comparable nano-Ag treated group at < 0.001 using Student’s -test. One way ANOVA was used to test the effect of different doses of Ag nanoparticles on the tested parameters.
(c)
Group
Treatment (dose mg/kg)
Tail length (px)
% DNA in tail
Tail moment
1
Negative control
7.51 ± 1.70
12.33 ± 1.33
1.01 ± 0.34
2
Cd(1.6)
21.36 ± 3.95
25.17 ± 1.72
5.84 ± 1.32
3
Ag(20)
15.35 ± 157
22.44 ± 1.67
4.33 ± 1.25
4
Ag(41)
22.89 ± 2.71
32.84 ± 3.53
7.37 ± 0.45
5
Ag(82)
34.45 ± 2.95
45.64 ± 3.09
15.75 ± 1.89
6
Ag(20) + Cd
23.65 ± 4.21
36.43 ± 9.79
7.58 ± 1.39
7
Ag(41) + Cd
34.05 ± 4.82
52.32 ± 4.15
17.71 ± 3.09
8
Ag(82) + Cd
54.92 ± 4.63
73.00 ± 4.48
37.08 ± 9.00
Groups 3, 4, and 5
= 74.9, < 0.001
= 81.6, < 0.001
= 100.5, < 0.001
Groups 6, 7, and 8
= 60.9, < 0.001
= 173.8, < 0.001
= 357.2, < 0.001
Results were expressed as mean ± SD. Statistically significantly different from the negative control group and statistically significantly different from the comparable nano-Ag treated group at < 0.05, < 0.01, and < 0.001 using Student’s -test. One way ANOVA was used to test the effect of different doses of Ag nanoparticles on the tested parameters.
