Label-Free Detection of Chondroitin Sulphate Proteoglycan 4 by a Polyaniline/Graphene Nanocomposite Functionalized Impedimetric Immunosensor

Figure 5

Analysis of cell membrane protein isolated from CSPG4-positive line MV3 by impedimetric immunosensor. (a) Impedance spectra of CSPG4 specific mAbD2.8.5 immobilized electrode before and after reaction with cell membrane protein; inset: impedance spectra of isotype mAbMk2-23 (does not recognize CSPG4) immobilized electrode before and after reaction with cell membrane proteins. (b) Histogram of normalized impedance signal changes.

(a)

(b)