Evaluation of the Osteoblast Behavior to PGA Textile Functionalized with RGD as a Scaffold for Bone Regeneration

Figure 4

Cell aggregates grown in OIM stain positive with ARS solution, indicating osteoblast differentiation and mineralization. Phase contrast micrographs at a total magnification of 10x. PGA-RGD-OIM at 4 days (a), 14 days (b), and 21 days (c). PGA-OIM at 4 days (d), 14 days (e), and 21 days (f). In the case for the aggregates in non-OIM at 4, 14, and 21 days showed no calcium deposition; PGA-RGD at 4 days (g), 14 days (h), and 21 days (i). PGA at 4 days (j), 14 days (k), and 21 days (l).

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