A Unique Protein Self-Assembling Nanoparticle with Significant Advantages in Vaccine Development and Production

Figure 4

SDS and Native PAGE of gD-NSP. (a) SDS-PAGE gel showing the overexpression of gD-NSP (MW 45.9 kD) in the crude bacterial lysate. (b) MW reference standard hen’s egg ovalbumin, 42.7 kD. Native PAGE electrophoresis gel of (c) horse spleen ferritin MW (450 kD). (d) gD-NSP MW (550 kD) in 50 mM Tris pH 7.5 300 mM NaCl and (e) gD-NSP in PBS, pH 7.4.