Research Article
Repair of Osteoporotic Bone Defects Using Adipose-Derived Stromal Cells and Umbilical Vein Endothelial Cells Seeded in Chitosan/Nanohydroxyapatite-P24 Nanocomposite Scaffolds
Figure 4
Immunofluorescence and immunohistochemistry. (a) Immunofluorescence of each group 4 weeks after surgery. Cells that appear green represent OCN-positive cells. Areas that appear red represent CD31-positive areas. (b) Immunohistochemistry of 4 and 8 weeks after surgery. Areas that appear dark brown represent CD31-positive areas, which indicate blood vessels (red arrow). The white areas are voids. (c) Mean fluorescent intensity of OCN-positive cells at 4 weeks. (d) Mean fluorescent intensity of CD31-positive cells at 4 weeks. (e) Quantification of CD31-positive cells in each group. Representative magnified images are at ×40 magnification. .
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