Research Article
Preparation, Characterization, and Evaluation of Rutin Nanocrystals as an Anticancer Agent against Head and Neck Squamous Cell Carcinoma Cell Line
Table 1
The primers utilized in real-time RT-PCR.
| | Gene name | Sequences (5-3) | Annealing temperature | Product size (bp) |
| | GAPDH (NCBI accession number NM_002046.7) | F: AGCCACATCGCTCAGACAC
R: GCCCAATACGACCAAATCC | 60 | 66 | | Bcl2 (NCBI accession number NM_000633.3) | F: CCTGTGGATGACTGAGTACC
R: GAGACAGCCAGGAGAAATCA | 55 | 128 | | Bax (NCBI accession number NM_138764.5) | F: TTTGCTTCAGGGTTTCATCCA
R: CTCCATGTTACTGTCCAGTTCGT | 55 | 151 | | Caspase 8 (NCBI accession number NM_001372051.1) | F: TCCAAATGCAAACTGGATGA
R: TCCCAGGATGACCCTCTTCT | 60 | 75 | | Caspase 9 (NCBI accession number NM_001229.5) | F: CCAGAGATTCGCAAACCAGAGG
R: GAGCACCGACATCACCAAATCC | 60 | 88 |
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