Ultrasensitive Detection of Lysozyme upon Conformational Change of DNA Duplex

Table 1

Comparison of proposed nanoaptasensor with other types of colorimetric assays and biosensors applied to lysozyme detection.

Assay formats and/or biosensor formats	Linear dynamic range	Detection limit	References
Assay based on electrostatic interaction with human serum albumin-modified AuNPs	18.7–561 nM	50 nM	[47]
Fluorescent aptasensor based on magnetic separation	0.56–12.3 nM	0.2 nM	[48]
Electrochemical impedance spectroscopy aptasensor based on electrodeposited AuNPs	0.1–500 pM	0.01 pM	[45]
Assay based on resonant Rayleigh scattering spectroscopy	– aM	7 aM	[8]
Signal-off architecture for electrochemical aptasensor	7–30 nM	0.45 nM	[49]
Aptasensor based on electron transfer	1–80 nM	0.5 nM	[26]
Digital microfluidic-based assay	40 fM-300 fM	44.6 fM	[46]
Electrochemical aptasensor utilizing target-induced turn-off of photosensitization	0.01-100 nM	2 pM	[27]
Assay based on capillary electrophoresis and inductively coupled plasma mass spectrometry	0–41.4 fM	3.89 aM	[51]
Nanoaptasensor based on electrostatic interaction of ssDNA-AuNPs and dsDNA-AuNPs	0.1–200 pM	50 fM	Our work