Magnesium–Magnetic Field Synergy Enhances Mouse Bone Marrow Mesenchymal Stem Cell Differentiation into Osteoblasts Via the MAGT1 Channel

Figure 4

Effect of MAGT1 gene silencing on osteogenesis. (a) MAGT1 siRNA was transfected into BMSCs with an efficiency of 42%, indicating successful transfection. (b) MAGT1 siRNA interference decreased ALP staining after 7 days, indicating that Mg²⁺-SMF induces osteogenesis via the MAGT1 gene. (c) MAGT1 siRNA interference decreased number of calcium nodules, suggesting that the MAGT1 gene plays a role in BMSCs osteogenesis and that Mg²⁺-SMF induces osteogenesis via the MAGT1 gene. (d) MAGT1 siRNA interference decreased the protein expression of Runx2, SP7, and ALP in BMSCs.

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