Magnesium–Magnetic Field Synergy Enhances Mouse Bone Marrow Mesenchymal Stem Cell Differentiation into Osteoblasts Via the MAGT1 Channel

Figure 5

Effect of CREB1 gene silencing on osteogenesis. (a) CREB1 siRNA was transfected into BMSCs with an efficiency of 38%, indicating successful transfection. (b) CREB1 siRNA interference decreased Alp staining, indicating that Mg2 + -SMF induces osteogenesis via the CREB1 gene. (c) CREB1 siRNA interference decreased the number of calcium nodules, suggesting that the CREB1 gene plays a role in BMSCs osteogenesis. (d) CREB1 siRNA interference decreased the protein expression of Runx2, SP7, and ALP in BMSCs.

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