Astragalus Polysaccharides Inhibit Pancreatic Cancer Progression by Downregulation of TLR4/NF-κB Signaling Pathway

Figure 1

APS inhibited viability and proliferation of PANC-1 cells. (a) CCK-8 assay was used to detect the effects of Astragalus polysaccharides (APS) on cell viability and proliferation in human normal pancreatic cell line HPC-Y5 and pancreatic cancer cell lines including PANC-1, SW1990, and AsPC-1. Various doses of APS (0, 1, 5, 10, 15, and 20 mg/mL) were treated to the cells and cultured for 24 h. (b) PANC-1 cell line STR identification. (c) CCK-8 assay was applied to explore the cell viability of different concentrations (0 (control), 1, 5, 10, 15, and 20 mg/mL) of APS treatment in PANC-1 cells. (d) EdU assay was used to examine the proliferation of APS on PANC-1 cells. (e) RNA-Seq analysis was used to detect the differentially expressed genes in PANC-1 cells treated with APS. (f) Western blot assay was performed to detect the expression of proliferation related proteins. (g) Protein interaction network of differentially expressed genes after APS treatment. The thicker the line, the stronger the interaction. , , and , compared with the control group. Scale  μm.

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