A Moderate Zinc Deficiency Does Not Alter Lipid and Fatty Acid Composition in the Liver of Weanling Rats Fed Diets Rich in Cocoa Butter or Safflower Oil

Table 4

Fatty acid composition (mol/100 mol) of liver phospholipids in male weanling rats fed high-fat diets differing in Zn content and fat source free choice or in restricted amounts for 4 wk.

Fatty acids	Diet groups					2 × 2 ANOVA: P level
	LZ-CB	HZ-CBR	LZ-SF	HZ-SFR	HZ-SF	Zn	Fat	Zn × fat
16:0						0.003	0.59	0.56
18:0						0.85	<0.001	0.14
SFAs						0.14	<0.001	0.12
18:1n-9						0.99	<0.001	0.056
MUFAs						0.59	<0.001	0.10
18:2n-6						0.53	<0.001	0.84
20:3n-6						0.001	0.52	0.050
20:4n-6						0.012	<0.001	0.40
22:4n-6					0.15	0.62	<0.001	0.65
n-6 PUFAs^6						0.022	<0.001	0.19
22:5n-3						0.42	0.028	0.73
22:6n-3^5						0.21	<0.001	0.34
n-3 PUFAs^5						0.17	<0.001	0.30
Δ^6 DS products^7						0.75	<0.001	0.52
Ratio n-6/n-3						0.017	<0.001	0.029
Relative UI						0.41	0.067	0.64

See footnote 1 of Table 2.
Values are means ± SD, . Labeled means in a row without a common letter differ by 1-factor ANOVA followed by Tukey’s multiple-comparison test, .
Bifactorial ANOVA of the LZ-CB, HZ-CBR, LZ-SF, and HZ-SFR diet groups.
Contain 16:1, 20:1n-9, and 22:1n-9.
1-factor and bifactorial ANOVA after logarithmic transformation of the data.
Contain 20:2n-6.
Total Δ⁶ desaturation products (mol per 100 mol PUFAs) contain 20:3n-6, 20:4n-6, 22:4n-6, 22:5n-3, and 22:n-3.
SFAs, saturated fatty acids; MUFAs, monounsaturated fatty acids; PUFAs, polyunsaturated fatty acids; UI, relative unsaturation index.