Western Diet Decreases Hepatic Drug Metabolism in Male LDLr^−/−ApoB^100/100 Mice

Figure 2

The effect of consumption of the Western diet (WD) on the probe protein-normalized activities of CYP enzymes in the liver microsomes of male LDLr^−/−ApoB^100/100 mice. The liver microsomes were prepared from the livers of eight months old mice; the animals were fed either with the WD or the chow diet for five months. CYP-oxidized reactions were determined with the indicated probe substrates: 7-ethoxyresorufin, 7-pentoxyresorufin, coumarin, 3-(4-trifluoromethoxyphenyl)-6-methoxycoumarin, 3-(3-benzyloxo)phenyl-7-methoxycoumarin, 3(3-fluoro-4-hydroxyphenyl)-6-methoxycoumarin, and 3-(3-methoxyphenyl)coumarin. , ; , ; and , WD vs. chow diet. The lines of the groups represent geometrical means with 95% confidence interval: (a) 7-ethoxyresorufin, (b) coumarin, (c) 7-pentoxyresorufin, (d) 3-(3-methoxyphenyl)-coumarin, (e) 3-(3-fluoro-4-hydroxyphenyl)-6-methoxycoumarin, (f) 3-(4-trifluoromethoxyphenyl)-6-methoxycoumarin, and (g) 3-(3-benzyloxo)phenyl-7-methoxycoumarin.

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