Research Article
Western Diet Decreases Hepatic Drug Metabolism in Male LDLr−/−ApoB100/100 Mice
Figure 3
The effect of the Western diet (WD) on the probe activities of conjugation enzymes in the liver cytosol or microsomes of male LDLr−/−ApoB100/100 mice. The liver microsome or cytosol samples were prepared from livers of eight months old mice; the animals were fed either with the WD or the chow diet for five months. Glucuronidation, sulfonation, and catechol (O)-methylation (COMT) activities determined with the indicated probe substrates. , p <0.05 , WD vs. chow diet. The lines of the groups represent geometrical means with 95% confidence interval: (a) glucuronidation of 7-hydroxy-4-trifluoromethylcoumarin, (b) glucuronidation of 3-(1H-1,2,4-triazol-1-yl)7-hydroxycoumarin, (c) sulfonation of 7-hydorxy-4-trifluoromethylcoumarin. (d) sulfonation of 3-(4-methylphenyl)-7-hydroxycoumarin, (e) cytosolic COMT, and (f) Microsomal COMT.
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