Western Diet Decreases Hepatic Drug Metabolism in Male LDLr^−/−ApoB^100/100 Mice

Figure 4

Multivariate analysis separated the Western diet (WD) fed male LDLr^−/−ApoB^100/100 mice into low xenobiotic metabolism activity group in contrast to mice fed with the chow diet. Biplots showing the first two latent components of the principal component analysis ((a) cumulative R²X = 0.99 and cumulative Q² = 0.58) and a partial least sum of squares discriminant analysis ((b) cumulative R²Y = 0.86 and cumulative Q² = 0.66) of CYP oxidation, glucuronidation, sulfonation, and COMT activities in liver of control and WD male LDLr^−/− ApoB^100/100 mice. Explanations of the symbols: C1 = ethoxyresorufin 7-(O)-deethylation, C2 = 7-hydroxylation of 3-(4-trifluoromethoxyphenyl)-6-methoxycoumarin, C3 = 7-hydroxylation of 3-(3-fluoro-4-hydroxyphenyl)-6-methoxycoumarin, C4 = coumarin 7-hydroxylation, C5 = pentoxyresorufin 7-(O)-depentylation, C6 = 7-hydroxylation of 3-(3-methoxyphenyl)coumarin, C7 = 7-(O)-demethylation of 3-(3-benzyloxo)phenyl-7-methoxycoumarin, G1 = HFC glucuronidation, G2 = 3-(1H-1, 2, 4-triazol-1-yl)-7-hydroxycoumarin glucuronidation, S1 = HFC sulfonation, S2 = 3-(4-methylphenyl)-7-hydroxycoumarin sulfonation, Cs = cytosol COMT, and Cm = microsomal COMT.

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