Study of Drug Target Identification and Associated Molecular Mechanisms for the Therapeutic Activity and Hair Follicle Induction of Two Ashwagandha Extracts Having Differential Withanolide Constitutions

Figure 4

ASH-Ext2 treatment upregulates β-catenin and TERT expressions in HFDPCs. HFDPCs were treated with ASH-Ext2 at 0.5, 1, and 2 μg/ml concentrations for 24 h and at 1 μg/ml concentration for 24 and 48 h. Data show (a, b) a dose-dependent and (c, d) a time-dependent increase in β-catenin expression in HFDPCs treated with ASH-Ext2. (e, f) β-Catenin expression levels in HFDPCs treated with rapamycin and withaferin (A). (g, h) A dose-dependent increase in TERT expression in HFDPCs treated with ASH-Ext2 at 0.5, 1, and 2 μg/ml concentrations for 24 h. Target protein expression was normalized to the samples’ loading control GAPDH and presented as fold change with respect to the control. For all data, N = 3. values are statistically significant ().

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