TJ-41 Induces Apoptosis and Potentiates the Apoptotic Effects of 5-FU in Breast Cancer Cell Lines

Figure 7

Effects of SP600125 (a JNK inhibitor), Z-IETD-FMK (a caspase-8 inhibitor), and Z-LEHD-FMK (a caspase-9 inhibitor) on TJ-41-induced apoptosis in MCF-7 and MDA-MB-231 breast cancer cell lines. BrCa cells were exposed to inhibitors for one hour prior to the addition of TJ-41 (48 hours). Percent of cells undergoing apoptosis (sub G₁) was measured by flow cytometry of propidium-iodide stained DNA. Data represents mean±SD of at least three experiments. Significant differences from untreated control are indicated: ^∗𝑃<.05, ^∗∗𝑃<.002, and ^∗∗∗𝑃<.001.