Use of H19 Gene Regulatory Sequences in DNA-Based Therapy for Pancreatic Cancer

Figure 7

DTA expression in tumors samples. Total RNA was extracted from frozen pancreatic tumors treated with DTA-H19 plasmid. DNase-treated RNA and nontreated RNA samples were analyzed by RT-PCR for DT-A transcript level. Lane 1: 100 bp marker, Lanes 2 and 4: PCR products from DNase-treated RNA, Lanes 3 and 5: PCR products from nontreated RNA, Lane 6: negative control, no cDNA is present in the reaction mixture. The upper panel shows the 468 bp DT-A product, and the lower panel shows the 𝛽-actin internal control.