Ganglioside GM3 Is Antiangiogenic in Malignant Brain Cancer

Figure 3

High-performance thin-layer chromatographic analysis of ganglioside distribution in the CT-2A astrocytoma: Control untransfected CT-2A (C), CT-2A transfected with empty vector alone (V), and CT-2A transfected with the antisense sequence to the GalNAc-T gene (T). Synthesized gangliosides appear as double bands due to ceramide structural heterogeneity. Analysis of synthesized gangliosides and standards (left lane) was as we described [7]. Knockdown of the GalNAc-T gene elevated GM3 content, while reducing GD1a content in the antisense T cells.