Bone Marrow Suppression by c-Kit Blockade Enhances Tumor Growth of Colorectal Metastases through the Action of Stromal Cell-Derived Factor-1
Figure 6
Western blot analysis of VEGF (a) and CXCR4 (b) expression within CT 26.WT tumors five days after tumor cell implantation. Animals were pretreated with anti-c-Kit (cKit-Ab) alone or additionally treated with a SDF-1 neutralizing antibody (cKit/SDF1-Ab). Animals treated with isotype-matched control antibodies served as controls (control). Quantitative analysis showed that VEGF expression was significantly higher after anti-c-Kit pretreatment than in control animals (a). Additional neutralization of SDF-1 had no further effect on the increased VEGF expression. CXCR4 was also significantly higher expressed after anti-c-Kit pretreatment compared to controls and was not further influenced by additional SDF-1 neutralization (b). Mean ± SEM; * versus control.
