lncRNA SNHG5 Modulates Endometrial Cancer Progression via the miR-25-3p/BTG2 Axis

Figure 6

miR-25-3p directly suppresses its target BTG2. (a) Predicted binding site of miR-25-3p in the 3′-UTR of BTG2 and the corresponding mutant. (b) Luciferase activity analyses of luciferase regulated by the binding site of miR-25-3p or its mutant after miR-25-3p overexpression. (c) Western blot analysis of BTG2 after miR-25-3p overexpression. (d) Analyses of BTG2 expression detected by qRT-PCR (top) and western blot assays (bottom) in KLE cells after overexpressing SNHG5 or both SNHG5 and miR-25-3p. (e) Analyses of BTG2 expression detected by qRT-PCR (top) and western blot assays (bottom) in HEC-1-B cells after overexpressing SNHG5 or both SNHG5 and miR-25-3p. ; .

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