GATA1 Promotes Gemcitabine Resistance in Pancreatic Cancer through Antiapoptotic Pathway
Figure 2
GATA1 promotes cell proliferation and gemcitabine resistance in vitro. (a) Western blot assay of GATA1 in PDAC and normal pancreatic cell lines. Relative expression of GATA1 in PDAC cell lines was quantified and listed in the table below along with the IC50 value. The cell viability curves of different PDAC cell lines were shown in the right panel. (b) Cell proliferation curves of CFPAC-1 cells stably infected with lentivirus carrying GATA1 (left panel), GATA1 shRNA, or GATA1 shRNA plus GATA1-R (right panel). The rescued cell line was established by reexpression of shRNA-resistant GATA1 (GATA1-R) in the GATA1 knockdown cells. GATA1 overexpression and knockdown effects in CFPAC-1 cells were validated by Western blot assay with β-actin as a loading control. (c) Representative images of colony formation assay in stable CFPAC-1 cells as established in (b). Relative colony numbers were quantified and compared by t test. (d) Cell viability assay of stable CFPAC-1 cells from (b). Cells were treated with a range of concentration of gemcitabine for 48 h before CCK8 test. (e) Representative images of colony formation assay in stable GATA1 knockdown CFPAC-1 cells. Cells were treated with gemcitabine (1 μM) for 48 h. Relative colony numbers were quantified and compared by t test.