Inhibition of JK184-Induced Cytoprotective Autophagy Potentiates JK184 Antitumor Effects in Breast Cancer

Figure 2

JK184 induces autophagy in BCa cells. (a) Western blot analysis of LC3, Beclin 1, Atg5, and p62/SQSTM1 in cells treated with the indicated concentrations of JK184 for 24 hours. (b) (Above) autophagy measured by acridine orange staining of cells treated with or without 4 nM JK184 for 24 hours. (Below) the total number of acidic vesicular organelles (AVO) per cell. Scale bar, 10 μm. (c-d) Endogenous LC3 puncta formed in BCa cells treated with DMSO or 4 nM JK184 for 24 hours. Scale bar, 10 μm. (e) Total number of endogenous LC3 puncta per cell.

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