Inhibition of JK184-Induced Cytoprotective Autophagy Potentiates JK184 Antitumor Effects in Breast Cancer

Figure 3

JK184 promotes autophagy flux in BCa cells. (a-b) Cells were transiently transfected with an RFP-GFP tandem fluorescent-tagged LC3 (RFP-GFP-LC3). Furthermore, cells were treated with 4 nm JK184 or in combination with 10 μM chloroquine (CQ) for 24 hours. Scale bar, 10 μm. (c-d) (Above) endogenous LC3 puncta analyzed by immunofluorescence in cells treated as in (a). (Below) total number of endogenous LC3 puncta per cell. , ; , . Scale bar, 10 μm.

(a)

(b)

(c)

(d)