Research Article
Role of the Exosome Secretion Machinery in Ovarian Carcinoma: In Vitro and In Vivo Models
Figure 4
(a) Migration assay. Below: extent of the wound open after 18 or 24 hours, respectively: (a) OVCAR8 control, (b) NSMASE2-KO (, n = 4), (c) TSAP6-KO (, n = 4), (d) RAB27A-KO (, n = 8), (e) RAB27B-KO (, n = 8). Above: quantification of experiment. Error bars represent SEM. (b) MMP activity assay. The width of the MMP9 gelatin consumed band is reduced in OVCAR8 NSMASE2 (), TSAP6 (), and RAB27A () KO cells, but not in RAB27B KO cells. Error bars represent SEM. (c) Invasion assay: cells infiltrating through matrigel filters were quantified in this assay. RAB27A/B-KO cells infiltrated less than the OVCAR8 control cells. and , respectively. Error bars represent SEM.
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