Correlation between FAK and EGF-Induced EMT in Colorectal Cancer Cells

Figure 2

FAK is involved in EGF-induced EMT in colorectal cancer cells and enhances cell migration and invasion abilities. (a, b) Cells were pretreated with FAK inhibitor PF-228 (10 μM) for 1 hour, and the phosphorylation levels of FAK and expression levels of E-cadherin and vimentin were significantly inhibited by the detection of Western blotting. (c, d) Cells were pretreated with FAK siRNA, and the phosphorylation levels of FAK and expression levels of E-cadherin and vimentin were significantly inhibited by the detection of Western blotting. (e, f) With the treatment of EGF and FAK inhibitor/FAK siRNA, the number of cell migration was detected by transwell assay. (g, h) With the treatment of EGF and FAK inhibitor/FAK siRNA, the number of cell invasion was detected by transwell assay.

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