Circulating Tumor DNA Using Tagged Targeted Deep Sequencing to Assess Minimal Residual Disease in Breast Cancer Patients Undergoing Neoadjuvant Chemotherapy

Figure 1

Diagram workflow of the planned sample collection and analyses: at diagnosis, tumor biopsies of patients presenting with invasive breast cancer and indication for NACT were analysed by MPS to identify somatic tumor-specific mutations and then confirmed by ddPCR and tTDS. Mutations in plasma were analysed by ddPCR at baseline, half treatment completed, before surgery, at 12 weeks, 24 weeks, 1 year after surgery, and every 24 weeks until 3 years of follow-up or upon relapse. tTDS was assessed at baseline on biopsies and plasma, as well as at relapse or last follow-up time point.