Upregulated Long Noncoding RNA UCA1 Enhances Warburg Effect via miR-203/HK2 Axis in Esophagal Cancer

Figure 3

UCA1 acts as a molecular sponge for miR-203 in EC cells. (a) The predicted binding sites between UCA1 and miR-203 were predicted by microRNA.org and Starbase v2.0. UCA1 mutated at the putative binding site. (b) Dual-luciferase reporter assay was performed to verify miR-203 was the target gene of UCA1. (c) Pearson’s correlation analysis between miR-203 and UCA1 expression in 60 EC samples (r = -0.5523, ). (d) miR-203 expression was measured in UCA1 knockdown EC cells by qRT-PCR. (e) UCA1 expression was detected after miR-203 overexpression by qRT-PCR. (f) The levels of UCA1 bound to Ago2 or IgG measured by qRT-PCR after RIP in EC cells with miR-203 mimics and miR-NC; , , and ; ns, no significance.

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