Upregulated Long Noncoding RNA UCA1 Enhances Warburg Effect via miR-203/HK2 Axis in Esophagal Cancer

Figure 4

UCA1 reverses the inhibitory effect of miR-203 on HK2. (a) Predicted miR-203 binding sites of HK2 and the corresponding mutant sequence. (b) The miR-203 targets the 3’UTR of HK2 analyzed by the luciferase reporter assays in EC109 cell. (c) The expression levels of HK2 decreased after miR-203 overexpression and increased by the miR-203 inhibitor. (d) Positive correlation between UCA1 and HK2 expression in EC tissues detected by Pearson’s correlation analysis (r = 0.4594, ). (e) Downregulated UCA1 inhibited the HK2 expression and ectopic expression of UCA1 (lv-UCA1) and increased the HK2 expression in EC109 cells. (f) Upregulation of UCA1 could reverse the inhibit effect of miR-203 on HK2 expression. (g) UCA1 silencing could reverse the miR-203 inhibitor-mediated upregulation of HK2. (h) Luciferase activity in EC109 cells transfected with luciferase reporters containing HK2 3’-UTR following transfection with sh-NC, sh-UCA1, anti-miR-203 + sh-NC, or anti-miR-293 + sh-UCA1. (i) Luciferase activity in EC109 cells transfected with luciferase reporters containing HK2 3’-UTR following transfection with lv-NC, lv-UCA1, miR-203-mimics + lv-NC, or miR-203-mimics + lv-UCA1. Data are shown as mean ± SEM; and .

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