Circ_0072088 Promotes Proliferation, Migration, and Invasion of Esophageal Squamous Cell Cancer by Absorbing miR-377

<div>Biological characteristics of circ_0072088 in ESCC cells. (a) The origin, composition, and length of circ_0072088. (b) Sanger sequencing of circ_0072088. The black arrow indicated the reverse splice site. (c) qRT-PCR was employed to examine the expression of circ_0072088 and linear ZFR mRNA in TE-13 cells before and after RNase R treatment. (d) Nuclear and cytoplasmic separation assay of RNA was performed to understand the distribution of circ_0072088 in TE-13 cells; 18S rRNA and U6 were taken as the positive controls for cytoplasmic and nuclear components, respectively. (e) FISH assay was performed to understand the distribution of circ_0072088 in TE-13 cells; 18S rRNA and U6 were taken as the positive controls for cytoplasmic and nuclear components, respectively (Scale bars: 20 <i>µ</i>m). <span class="nowrap"><svg height="10.7395pt" id="M10" style="vertical-align:-0.6370001pt" version="1.1" viewbox="-0.0498162 -10.1025 56.4993 10.7395" width="56.4993pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M486 158C486 177 478 202 466 220C413 228 386 236 336 262C386 288 413 297 466 304C478 323 486 347 485 366C470 376 444 381 422 380C389 338 368 319 321 288C323 345 329 372 349 422C339 442 322 461 305 470C289 461 271 442 262 422C281 372 287 345 290 288C243 319 222 338 189 380C167 381 142 376 125 366C125 347 133 322 145 304C198 296 225 288 275 262C225 236 198 227 145 220C133 201 125 177 126 158C141 148 167 143 189 144C222 186 243 205 290 236C288 179 282 152 262 102C272 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Journal of Oncology

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Figure 2

Figure 2: Circ_0072088 Promotes Proliferation, Migration, and Invasion of Esophageal Squamous Cell Cancer by Absorbing miR-377 