Saponin Facilitates Anti-Robo1 Immunotoxin Cytotoxic Effects on Maxillary Sinus Squamous Cell Carcinoma

Figure 1

The dose-dependent cytotoxic effect of saponin on HSQ-89 cells (a). The effect of saponin on the viability of IT-Robo1 or IT-NC treated HSQ-89 cells (b, c) HSQ-89 cells were seeded at 20,000 cells/well of a 96-well plate and incubated with various concentrations (0.054 pM∼4.2 nM) of either IT-Robo1 or IT-NC together with 3.5 μg/mL of saponin for 48 h. The treatment protocol for (b) continuous IT-saponin or (c) the washout experiment is designated under the respective panels. The filled circle with the solid line indicates IT-Robo1 with saponin, and the open circle with the dotted line indicates IT-NC with saponin.

(a)

(b)

(c)