Hsa-miR-330-5p Aggravates Thyroid Carcinoma via Targeting FOXE1

Figure 3

FOXE1 is a direct target of miR-330-5p in TC. (a) Schematic representation of the procedure for the identification of putative miR-330-5p target genes. As described in the Methods section, the analysis derives from an integration of miR-330-5p targets predicted by TargetScan, miRwalk, and ENCORI software. (b) Bioinformatic analysis of the predicted binding sites of FOXE1 and miR-330-5p. (c) Luciferase reporter assay detected the luciferase activity of reporter vector containing WT or mutant form of FOXE1 3′-UTR along with NC mimic or miR-330-5p mimic. (d) qRT-PCR and (e) western blot analysis showed that FOXE1 was upregulated in TPC-1 and K-1 cells with miR-330-5p inhibitor transfection. versus NC inhibitor. (f-g) qRT-PCR and western blot assay showed that the expression levels of FOXE1 were decreased in TPC-1 and K-1 cells. Values are mean ± SD. and versus normal cells.

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